Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.
The implementation of high-throughput (HTP) cloning and expression screening in Escherichia coli by 14 laboratories in the Structural Proteomics In Europe (SPINE) consortium is described. Cloning efficiencies of greater than 80% have been achieved for the three non-ligation-based cloning techniques...
Asıl Yazarlar: | Alzari, P, Berglund, H, Berrow, N, Blagova, E, Busso, D, Cambillau, C, Campanacci, V, Christodoulou, E, Eiler, S, Fogg, M, Folkers, G, Geerlof, A, Hart, D, Haouz, A, Herman, MD, Macieira, S, Nordlund, P, Perrakis, A, Quevillon-Cheruel, S, Tarandeau, F, van Tilbeurgh, H, Unger, T, Luna-Vargas, M, Velarde, M, Willmanns, M |
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Materyal Türü: | Journal article |
Dil: | English |
Baskı/Yayın Bilgisi: |
2006
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