Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.

The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has dep...

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Bibliographic Details
Main Authors: Keates, T, Cooper, C, Savitsky, P, Allerston, C, Phillips, C, Hammarström, M, Daga, N, Berridge, G, Mahajan, P, Burgess-Brown, N, Müller, S, Gräslund, S, Gileadi, O
Format: Journal article
Language:English
Published: 2012