Following protein folding in real time using NMR spectroscopy.
The refolding of apo bovine alpha-lactalbumin has been monitored in real time by NMR spectroscopy following rapid in situ dilution of a chemically denatured state. By examining individual resonances in the time-resolved NMR spectra, the native state has been shown to emerge in a cooperative manner f...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1995
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