Single-molecule FRET analysis of protein-DNA complexes.
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presen...
Main Authors: | , , , |
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Format: | Journal article |
Language: | English |
Published: |
2009
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