Single-molecule FRET analysis of protein-DNA complexes.

We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presen...

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Bibliographic Details
Main Authors: Heilemann, M, Hwang, L, Lymperopoulos, K, Kapanidis, A
Format: Journal article
Language:English
Published: 2009