Single-molecule FRET analysis of protein-DNA complexes.
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presen...
| Autores principales: | , , , |
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| Formato: | Journal article |
| Lenguaje: | English |
| Publicado: |
2009
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| _version_ | 1826262432099598336 |
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| author | Heilemann, M Hwang, L Lymperopoulos, K Kapanidis, A |
| author_facet | Heilemann, M Hwang, L Lymperopoulos, K Kapanidis, A |
| author_sort | Heilemann, M |
| collection | OXFORD |
| description | We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented. |
| first_indexed | 2024-03-06T19:36:04Z |
| format | Journal article |
| id | oxford-uuid:1f17580e-571c-4669-9427-3ee4b8d659c7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:36:04Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:1f17580e-571c-4669-9427-3ee4b8d659c72022-03-26T11:19:57ZSingle-molecule FRET analysis of protein-DNA complexes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:1f17580e-571c-4669-9427-3ee4b8d659c7EnglishSymplectic Elements at Oxford2009Heilemann, MHwang, LLymperopoulos, KKapanidis, AWe present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented. |
| spellingShingle | Heilemann, M Hwang, L Lymperopoulos, K Kapanidis, A Single-molecule FRET analysis of protein-DNA complexes. |
| title | Single-molecule FRET analysis of protein-DNA complexes. |
| title_full | Single-molecule FRET analysis of protein-DNA complexes. |
| title_fullStr | Single-molecule FRET analysis of protein-DNA complexes. |
| title_full_unstemmed | Single-molecule FRET analysis of protein-DNA complexes. |
| title_short | Single-molecule FRET analysis of protein-DNA complexes. |
| title_sort | single molecule fret analysis of protein dna complexes |
| work_keys_str_mv | AT heilemannm singlemoleculefretanalysisofproteindnacomplexes AT hwangl singlemoleculefretanalysisofproteindnacomplexes AT lymperopoulosk singlemoleculefretanalysisofproteindnacomplexes AT kapanidisa singlemoleculefretanalysisofproteindnacomplexes |