Site-selective glycosylation of subtilisin Bacillus lentus causes dramatic increases in esterase activity.
Using site directed mutagenesis combined with chemical modification, we have developed a general and versatile method for the glycosylation of proteins which is virtually unlimited in the scope of proteins and glycans that may be conjugated and in which the site of glycosylation and the nature of th...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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