Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplico...

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Bibliografiske detaljer
Main Authors:	El-Sagheer, A, Brown, T
Format:	Journal article
Sprog:	English
Udgivet:	2009

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