Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplico...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς:	El-Sagheer, A, Brown, T
Μορφή:	Journal article
Γλώσσα:	English
Έκδοση:	2009

Παρόμοια τεκμήρια

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation
ανά: Chen, J, κ.ά.
Έκδοση: (2018)

A triazole linkage that mimics the DNA phosphodiester group in living systems
ανά: El-Sagheer, A, κ.ά.
Έκδοση: (2015)

Effect of Molecular Crowding on DNA Polymerase Reactions along Unnatural DNA Templates
ανά: Shuntaro Takahashi, κ.ά.
Έκδοση: (2020-09-01)

Optimisation of an asymmetric polymerase chain reaction assay for the amplification of single-stranded DNA from Wuchereria bancrofti for electrochemical detection
ανά: Vasuki Venkatesan, κ.ά.
Έκδοση: (2013-09-01)

Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
ανά: Andrew F. Gardner, κ.ά.
Έκδοση: (2019-04-01)

Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing
ανά: Chen, X, κ.ά.
Έκδοση: (2014)

Efficient RNA synthesis by in vitro transcription of a triazole-modified DNA template.
ανά: el-Sagheer, A, κ.ά.
Έκδοση: (2011)

Simplified Method for Preservation and Polymerase Chain Reaction-amplification of Trypanosoma cruzi DNA in Human Blood
ανά: Patricia L Dorn, κ.ά.
Έκδοση: (1997-03-01)

Nucleic acid amplification: Alternative methods of polymerase chain reaction
ανά: Md Fakruddin, κ.ά.
Έκδοση: (2013-01-01)

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification
ανά: Konstantin B. Ignatov, κ.ά.
Έκδοση: (2014-08-01)

DNA extraction and polymerase chain reaction
ανά: Nalini Gupta
Έκδοση: (2019-01-01)

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis
ανά: Dwi Veni Endarwati, κ.ά.
Έκδοση: (2024-06-01)

Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
ανά: AL Cândido, κ.ά.
Έκδοση: (2000-04-01)

Diagnosis of Streptococcus pneumoniae meningitis by polymerase chain reaction amplification of the gene for pneumolysin
ανά: Juliana de A Matos, κ.ά.
Έκδοση: (2006-08-01)

Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
ανά: Susana Pedraza-Díaz, κ.ά.
Έκδοση: (2001-02-01)

Rapid and efficient DNA strand cross-linking by click chemistry.
ανά: Kocalka, P, κ.ά.
Έκδοση: (2008)

Cyclisation reactions for the synthesis of natural and unnatural piperidines
ανά: Barat, Viktor
Έκδοση: (2017)

HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.
ανά: Wordsworth, B, κ.ά.
Έκδοση: (1990)

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
ανά: Taemaitree, L, κ.ά.
Έκδοση: (2019)

Synthesis and Properties of Oligonucleotides Containing LNA-Sulfamate and Sulfamide Backbone Linkages
ανά: Zengin Kurt, B, κ.ά.
Έκδοση: (2024)

Searching for the ideal triazole: investigating the 1,5-triazole as a charge neutral DNA backbone mimic
ανά: Baker, YR, κ.ά.
Έκδοση: (2019)

Optimisation of reverse transcription-polymerase chain reaction for double stranded RNA extraction / Jamiila Ismail
ανά: Ismail, Jamiila
Έκδοση: (2007)

DNA polymerase delta-dependent repair of DNA single strand breaks containing 3'-end proximal lesions.
ανά: Parsons, J, κ.ά.
Έκδοση: (2007)

Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides
ανά: A. Amarasinghe, κ.ά.
Έκδοση: (2021-04-01)

Detection of Mycobacterium leprae DNA from archaeological skeletal remains in Japan using whole genome amplification and polymerase chain reaction.
ανά: Koichi Suzuki, κ.ά.
Έκδοση: (2010-01-01)

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
ανά: Lapatrada Taemaitree, κ.ά.
Έκδοση: (2019-04-01)

“Unnatural, unnatural, unnatural, unnatural unnatural” . . . but real? The Toolbox Murders (Dennis Donnelly, 1978) and the Exploitation of True Story Adaptations
ανά: Wickham Clayton
Έκδοση: (2016-07-01)

Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase
ανά: G.J. Hafner, κ.ά.
Έκδοση: (2001-04-01)

Structure and dynamics of triazole-linked DNA: biocompatibility explained.
ανά: Dallmann, A, κ.ά.
Έκδοση: (2011)

The polymerase chain reaction /
ανά: Mullis, Kary B., κ.ά.
Έκδοση: (1994)

Polymerase Chain Reaction - Single Strand Conformation Polymorphism (PCR-SSCP) analysis of goat as2-casein
ανά: A. Caroli, κ.ά.
Έκδοση: (2010-04-01)

Use of the Restriction Enzyme AvaI and Exo− Bst Polymerase in Strand Displacement Amplification
ανά: Maria A. Milla, κ.ά.
Έκδοση: (1998-03-01)

Sequencing of simple sequence repeat anchored polymerase chain reaction amplification products of Biomphalaria glabrata
ανά: Roberta L Caldeira, κ.ά.
Έκδοση: (2002-10-01)

Assessment of Cost-Efficient Thermocycler Prototype for Polymerase Chain Reaction and Loop-Mediated Isothermal Amplification
ανά: Kenny Lischer, κ.ά.
Έκδοση: (2021-12-01)

Improved diagnostic potential of polymerase chain reaction by amplification of multiple gene targets in osteoarticular tuberculosis
ανά: Gopinath Palanisamy, κ.ά.
Έκδοση: (2017-01-01)

A microheater on polyimide substrate for hand-held realtime microfluidic polymerase chain reaction amplification
ανά: Dae-Sik Lee, κ.ά.
Έκδοση: (2019-10-01)

Impact of Amplification Efficiency Approaches on Telomere Length Measurement via Quantitative-Polymerase Chain Reaction
ανά: Waylon J. Hastings, κ.ά.
Έκδοση: (2021-09-01)

Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.
ανά: Guillaume Roux, κ.ά.
Έκδοση: (2019-01-01)

Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin (AMELX/AMELY) gene
ανά: P. Gokulakrishnan, κ.ά.
Έκδοση: (2012-01-01)

Novel Strategies to Optimize the Amplification of Single-Stranded DNA
ανά: Atef Nehdi, κ.ά.
Έκδοση: (2020-05-01)