Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplico...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron: El-Sagheer, A, Brown, T
Fformat: Journal article
Iaith:English
Cyhoeddwyd: 2009