Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplico...

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Bibliografische gegevens
Hoofdauteurs: El-Sagheer, A, Brown, T
Formaat: Journal article
Taal:English
Gepubliceerd in: 2009