GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System.

A key step in the production of recombinant membrane proteins for structural studies is the optimization of protein yield and quality. One commonly used approach is to fuse the protein to green fluorescent protein (GFP), enabling expression to be tracked without the need to purify the protein. Combi...

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Bibliographic Details
Main Authors: Hu, N, Rada, H, Rahman, N, Nettleship, J, Bird, L, Iwata, S, Drew, D, Cameron, A, Owens, R
Format: Journal article
Language:English
Published: Humana Press Inc. 2015