Over-expression, purification, and characterization of recombinant human arylamine N-acetyltransferase 1.

Human arylamine N-acetyltransferase 1 (NAT1) has been overexpressed in E. coli as a mutant dihydrofolic acid reductase (DHFR) fusion protein with a thrombin sensitive linker. An initial DEAE anion-exchange chromatography resulted in partial purification of the fusion protein. The fusion protein was...

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Bibliographic Details
Main Authors: Wang, H, Vath, G, Kawamura, A, Bates, C, Sim, E, Hanna, P, Wagner, C
Format: Journal article
Language:English
Published: 2005