Over-expression, purification, and characterization of recombinant human arylamine N-acetyltransferase 1.
Human arylamine N-acetyltransferase 1 (NAT1) has been overexpressed in E. coli as a mutant dihydrofolic acid reductase (DHFR) fusion protein with a thrombin sensitive linker. An initial DEAE anion-exchange chromatography resulted in partial purification of the fusion protein. The fusion protein was...
Main Authors: | Wang, H, Vath, G, Kawamura, A, Bates, C, Sim, E, Hanna, P, Wagner, C |
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Format: | Journal article |
Language: | English |
Published: |
2005
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