Visualization of focal sites of transcription within human nuclei.
HeLa cells were encapsulated in agarose microbeads, permeabilized and incubated with Br-UTP in a 'physiological' buffer; then sites of RNA synthesis were immunolabelled using an antibody that reacts with Br-RNA. After extending nascent RNA chains by < 400 nucleotides in vitro, appro...
| Main Authors: | , , , |
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| Format: | Journal article |
| Sprog: | English |
| Udgivet: |
1993
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