A comparison of exogenous promoter activity at the ROSA26 locus using a (Phi)iC31 integrase mediated cassette exchange approach in mouse ES cells.
The activities of nine ubiquitous promoters (ROSA26, CAG, CMV, CMVd1, UbC, EF1(Alpha), PGK, chicken (Beta)-actin and MC1) have been quantified and compared in mouse embryonic stem cells. To avoid the high variation in transgene expression which results from uncontrolled copy number and chromosomal p...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Public Library of Science
2011
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