Increasing knock-in efficiency in mouse zygotes by transient hypothermia

Increasing knock-in efficiency in mouse zygotes by transient hypothermia

Integration of a point mutation to correct or edit a gene requires the repair of the CRISPR-Cas9-induced double-strand break by homology directed repair (HDR). This repair pathway is more active in late S and G2 phases of the cell cycle, whereas the competing pathway of non-homologous end joining (N...

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Autors principals:	Bouchareb, A, Biggs, D, Alghadban, S, Preece, C, Davies, B
Format:	Journal article
Idioma:	English
Publicat:	Mary Ann Liebert 2024

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