Increasing knock-in efficiency in mouse zygotes by transient hypothermia
Integration of a point mutation to correct or edit a gene requires the repair of the CRISPR-Cas9-induced double-strand break by homology directed repair (HDR). This repair pathway is more active in late S and G2 phases of the cell cycle, whereas the competing pathway of non-homologous end joining (N...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Mary Ann Liebert
2024
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