A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm
Centrioles are small barrel-shaped structures that form centrosomes and cilia [1]. Centrioles assemble around a central cartwheel comprising the Sas-6 and Ana2/STIL proteins. The amino termini of nine Sas-6 dimers form a central hub of ∼12 nm radius from which nine dimer spokes radiate, placing the...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
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Cell Press
2017
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| _version_ | 1826263746872344576 |
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| author | Gartenmann, L Wainman, A Qurashi, M Kaufmann, R Schubert, S Raff, J Dobbie, I |
| author_facet | Gartenmann, L Wainman, A Qurashi, M Kaufmann, R Schubert, S Raff, J Dobbie, I |
| author_sort | Gartenmann, L |
| collection | OXFORD |
| description | Centrioles are small barrel-shaped structures that form centrosomes and cilia [1]. Centrioles assemble around a central cartwheel comprising the Sas-6 and Ana2/STIL proteins. The amino termini of nine Sas-6 dimers form a central hub of ∼12 nm radius from which nine dimer spokes radiate, placing the Sas-6 carboxyl termini at the outer edge of the ∼60 nm radius cartwheel [2]. Several centriole proteins are distributed in a toroid around the cartwheel, and super-resolution light microscopy studies have measured the average radii of these ∼100–200 nm radius toroids with a ‘precision’ — or standard deviation (s.d. or 1σ) — of ±∼10–40 nm. The organization of Ana2/STIL within the cartwheel, however, has not been resolvable. Here, we develop methods to calculate the average toroidal radius of centriolar proteins in the ∼20–60 nm range with a s.d. of just ±∼4–5 nm, revealing that the amino and carboxyl termini of Ana2 are located in the outer cartwheel region. |
| first_indexed | 2024-03-06T19:56:44Z |
| format | Journal article |
| id | oxford-uuid:25de3a8b-c714-4ea1-abf4-bf6f37e14f5d |
| institution | University of Oxford |
| last_indexed | 2024-03-06T19:56:44Z |
| publishDate | 2017 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:25de3a8b-c714-4ea1-abf4-bf6f37e14f5d2022-03-26T11:57:57ZA combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nmJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:25de3a8b-c714-4ea1-abf4-bf6f37e14f5dSymplectic Elements at OxfordCell Press2017Gartenmann, LWainman, AQurashi, MKaufmann, RSchubert, SRaff, JDobbie, ICentrioles are small barrel-shaped structures that form centrosomes and cilia [1]. Centrioles assemble around a central cartwheel comprising the Sas-6 and Ana2/STIL proteins. The amino termini of nine Sas-6 dimers form a central hub of ∼12 nm radius from which nine dimer spokes radiate, placing the Sas-6 carboxyl termini at the outer edge of the ∼60 nm radius cartwheel [2]. Several centriole proteins are distributed in a toroid around the cartwheel, and super-resolution light microscopy studies have measured the average radii of these ∼100–200 nm radius toroids with a ‘precision’ — or standard deviation (s.d. or 1σ) — of ±∼10–40 nm. The organization of Ana2/STIL within the cartwheel, however, has not been resolvable. Here, we develop methods to calculate the average toroidal radius of centriolar proteins in the ∼20–60 nm range with a s.d. of just ±∼4–5 nm, revealing that the amino and carboxyl termini of Ana2 are located in the outer cartwheel region. |
| spellingShingle | Gartenmann, L Wainman, A Qurashi, M Kaufmann, R Schubert, S Raff, J Dobbie, I A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm |
| title | A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm |
| title_full | A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm |
| title_fullStr | A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm |
| title_full_unstemmed | A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm |
| title_short | A combined 3D-SIM/SMLM approach allows centriole proteins to be localised with a precision of ~4-5nm |
| title_sort | combined 3d sim smlm approach allows centriole proteins to be localised with a precision of 4 5nm |
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