Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis

Förster resonance energy transfer (FRET) detected via fluorescence lifetime imaging microscopy (FLIM) and global analysis provide a way in which protein-protein interactions may be spatially localized and quantified within biological cells. The FRET efficiency and proportion of interacting molecules...

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Main Authors: Barber, P, Ameer-Beg, S, Gilbey, J, Carlin, L, Keppler, M, Ng, T, Vojnovic, B
格式: Journal article
语言:English
出版: 2009