Crystallization of the NADP(+)-dependent glutamate dehydrogenase from Escherichia coli.

The NADP(+)-dependent hexameric glutamate dehydrogenase from Escherichia coli has been crystallized as the apo-enzyme and also in the presence of its substrates 2-oxoglutarate, glutamate or NADP+, using either pulsed equilibrium microdialysis, or the hanging drop method of vapour diffusion. Three no...

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Bibliographic Details
Main Authors: Korber, F, Rizkallah, P, Attwood, T, Wootton, J, McPherson, M, North, A, Geddes, A, Abeysinghe, I, Baker, P, Dean, J
Format: Journal article
Language:English
Published: 1993