Yield, solubility and conformational quality of soluble proteins are not simultaneously favored in recombinant Escherichia coli.
Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational qual...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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