A protein biosensor that relies on bending of single DNA molecules.
A "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demo...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1826264488176779264 |
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| author | Crawford, R Kelly, D Kapanidis, A |
| author_facet | Crawford, R Kelly, D Kapanidis, A |
| author_sort | Crawford, R |
| collection | OXFORD |
| description | A "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA-bending proteins. |
| first_indexed | 2024-03-06T20:08:36Z |
| format | Journal article |
| id | oxford-uuid:29c66517-1305-4188-b1e8-dfa89c4c0044 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:08:36Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:29c66517-1305-4188-b1e8-dfa89c4c00442022-03-26T12:21:04ZA protein biosensor that relies on bending of single DNA molecules.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:29c66517-1305-4188-b1e8-dfa89c4c0044EnglishSymplectic Elements at Oxford2012Crawford, RKelly, DKapanidis, AA "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA-bending proteins. |
| spellingShingle | Crawford, R Kelly, D Kapanidis, A A protein biosensor that relies on bending of single DNA molecules. |
| title | A protein biosensor that relies on bending of single DNA molecules. |
| title_full | A protein biosensor that relies on bending of single DNA molecules. |
| title_fullStr | A protein biosensor that relies on bending of single DNA molecules. |
| title_full_unstemmed | A protein biosensor that relies on bending of single DNA molecules. |
| title_short | A protein biosensor that relies on bending of single DNA molecules. |
| title_sort | protein biosensor that relies on bending of single dna molecules |
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