Major signal increase in fluorescence microscopy through dark-state relaxation.
We report a substantial signal gain in fluorescence microscopy by ensuring that transient molecular dark states with lifetimes >1 micros, such as the triplet state relax between two molecular absorption events. For GFP and Rhodamine dye Atto532, we observed a 5-25-fold increase in total fluor...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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