High-throughput class I HLA genotyping using fluorescence resonance energy transfer (FRET) probes and sequence-specific primer-polymerase chain reaction (SSP-PCR).

We have developed a semi-automated HLA class I typing system utilising TET/TAMRA-labelled fluorescence resonance energy transfer (FRET) hydrolysis probes. The results from 87 individuals are in full concordance with serology and conventional gel-based systems. This assay replaces labour-intensive co...

Deskribapen osoa

Xehetasun bibliografikoak
Egile Nagusiak: Gelsthorpe, A, Wells, R, Lowe, A, Tonks, S, Bodmer, J, Bodmer, W
Formatua: Journal article
Hizkuntza:English
Argitaratua: 1999