Studies on the activity of the hypoxia-inducible-factor hydroxylases using an oxygen consumption assay.
The activity and levels of the metazoan HIF (hypoxia-inducible factor) are regulated by its hydroxylation, catalysed by 2OG (2-oxoglutarate)- and Fe(II)-dependent dioxygenases. An oxygen consumption assay was developed and used to study the relationship between HIF hydroxylase activity and oxygen co...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2007
|