Cellular gels. Purifying and mapping long DNA molecules.
Long DNA molecules of greater than 10(5) bp (0.1 Mbp) are easily broken by pipetting. Therefore, chromosomal DNA is generally isolated after embedding cells in a protective coat of agarose. The embedded DNA can then be cut into long pieces and fractionated on gels using pulsed fields, but these piec...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1991
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