High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording

The performance of a method is assessed which allows for the spatiotemporal tracking of single dye-labeled molecules during two-dimensional (2D) diffusional transits through the focal area of a modified confocal microscope. In addition to facilitating the observation of molecular diffusion paths at...

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Bibliographic Details
Main Authors: Sahl, S, Leutenegger, M, Hell, S, Eggeling, C
Format: Journal article
Language:English
Published: Wiley 2014