High-resolution tracking of single-molecule diffusion in membranes by confocalized and spatially differentiated fluorescence photon stream recording
The performance of a method is assessed which allows for the spatiotemporal tracking of single dye-labeled molecules during two-dimensional (2D) diffusional transits through the focal area of a modified confocal microscope. In addition to facilitating the observation of molecular diffusion paths at...
Hauptverfasser: | Sahl, S, Leutenegger, M, Hell, S, Eggeling, C |
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Format: | Journal article |
Sprache: | English |
Veröffentlicht: |
Wiley
2014
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