Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
5-Azidomethyl dUTP and two 5-trans-cyclooctene dUTPs with different linkers between the TCO and the uracil base have been incorporated into DNA by primer extension, reverse-transcription and PCR amplification. For azidomethyl dUTP the PCR reaction was successful even when the modified dUTP was not s...
मुख्य लेखकों: | Ren, X, El-Sagheer, A, Brown, T |
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स्वरूप: | Journal article |
भाषा: | English |
प्रकाशित: |
Royal Society of Chemistry
2015
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समान संसाधन
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