Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system.

IpaD, the putative needle-tip protein of the Shigella flexneri type III secretion system, has been overexpressed and purified. Crystals were grown of the native protein in space group P2(1)2(1)2(1), with unit-cell parameters a = 55.9, b = 100.7, c = 112.0 A, and data were collected to 2.9 A resoluti...

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Bibliographic Details
Main Authors: Johnson, S, Roversi, P, Espina, M, Deane, J, Birket, S, Picking, W, Blocker, A, Lea, S
Format: Journal article
Language:English
Published: 2006