Developing liquid chromatography-mass spectrometry strategies for investigating energy metabolism with application to isocitrate dehydrogenase mutations in cancer

Isocitrate dehydrogenases (IDHs) act in the tricarboxylic acid (TCA) cycle to catalyse the conversion of isocitrate to 2-oxoglutarate (2-OG) with concomitant production of NADH and/or NADPH. In humans, mutations causing IDH1 and IDH2 substitutions have been found during DNA sequencing of human gliob...