Quantitation of protein expression in a cell-free system: Efficient detection of yields and 19F NMR to identify folded protein.
We have developed an efficient and novel filter assay method, involving radioactive labelling and imaging, to quantify the expression of soluble proteins from a cell-free translation system. Here this method is combined with the conformational sensitivity of 19F NMR to monitor the folded state of th...
| Main Authors: | , , , , |
|---|---|
| 格式: | Journal article |
| 语言: | English |
| 出版: |
2005
|