Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine
We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...
| Egile Nagusiak: | , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
Oxford University Press
2011
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| Gaiak: |