Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

Бусад хувилбаруудыг харуулах (1)

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид:	Hua, H, Kearsey, S
Формат:	Journal article
Хэл сонгох:	English
Хэвлэсэн:	Oxford University Press 2011
Нөхцлүүд:	Zoological sciences

Ижил төстэй зүйлс

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine.
-н: Hua, H, зэрэг
Хэвлэсэн: (2011)

GINS inactivation phenotypes reveal two pathways for chromatin association of replicative α and ε DNA polymerases in fission yeast
-н: Pai, C, зэрэг
Хэвлэсэн: (2009)

Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding
-н: Gregan, J, зэрэг
Хэвлэсэн: (2003)

Nuclear distribution and chromatin association of DNA polymerase α-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast
-н: Yang, X, зэрэг
Хэвлэсэн: (2005)

Rapid regulation of protein activity in fission yeast
-н: Bøe, C, зэрэг
Хэвлэсэн: (2008)

Essential role of MCM proteins in premeiotic DNA replication
-н: Lindner, K, зэрэг
Хэвлэсэн: (2002)

DNAReplication: a database of information and resources for the eukaryotic DNA replication community
-н: Cotterill, S, зэрэг
Хэвлэсэн: (2009)

Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies
-н: Suzanne B. Buck, зэрэг
Хэвлэсэн: (2008-06-01)

Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2′-deoxyuridine and 5-ethynyl-2′-deoxycytidine
-н: Anna Ligasová, зэрэг
Хэвлэсэн: (2016-01-01)

Actively replicating gut bacteria identified by 5-ethynyl-2’-deoxyuridine (EdU) click chemistry and cell sorting
-н: Eve T. Beauchemin, зэрэг
Хэвлэсэн: (2023-12-01)

MCM2-7 proteins are essential components of prereplicative complexes that accumulate cooperatively in the nucleus during G1-phase and are required to establish, but not maintain, the S-phase checkpoint
-н: Labib, K, зэрэг
Хэвлэсэн: (2001)

Regulation of DNA replication during meiosis in fission yeast
-н: Hua, H
Хэвлэсэн: (2012)

Synonymous substitution rates predict HIV disease progression as a result of underlying replication dynamics
-н: Lemey, P, зэрэг
Хэвлэсэн: (2007)

Yeast forms dominate fungal diversity in the deep oceans
-н: Bass, D, зэрэг
Хэвлэсэн: (2007)

Effect of habitat quality upon reintroduction success in water voles: evidence from a replicated experiment
-н: Moorhouse, T, зэрэг
Хэвлэсэн: (2009)

The evolution of social behaviour: the effect of mating system and social structure in the European badger Meles meles
-н: Dugdale, H
Хэвлэсэн: (2007)

Multiplexed SNP typing of ancient DNA clarifies the origin of Andaman mtDNA haplogroups amongst South Asian tribal populations
-н: Endicott, P, зэрэг
Хэвлэсэн: (2006)

Disease and Ethiopian wolves (Canis simensis): trialling the feasibility of oral vaccination against rabies
-н: Gordon, C, зэрэг
Хэвлэсэн: (2013)

Conditional inactivation of replication proteins in fission yeast using hormone-binding domains.
-н: Pai, C, зэрэг
Хэвлэсэн: (2012)

Conditional inactivation of replication proteins in fission yeast using hormone-binding domains
-н: Pai, C, зэрэг
Хэвлэсэн: (2012)

Avian malaria in the montane tropics
-н: Daly, B
Хэвлэсэн: (2013)

Social evolution in class-structured populations
-н: Rodrigues, A, зэрэг
Хэвлэсэн: (2014)

Developments in social evolution and virulence in parasites
-н: Leggett, H, зэрэг
Хэвлэсэн: (2014)

GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.
-н: Pai, C, зэрэг
Хэвлэсэн: (2009)

Studies on Aedes polynesiensis introgression and ecology to facilitate lymphatic filariasis control
-н: Hapairai, L
Хэвлэсэн: (2013)

Genome evolution in Streptococcus pneumoniae.
-н: Wyres, K
Хэвлэсэн: (2012)

Introgression and the current status of the Scottish wildcat (Felis silvestris silvestris)
-н: Kilshaw, K
Хэвлэсэн: (2015)

Comparative genomics of vertebrate Fox cluster loci
-н: Wotton, K, зэрэг
Хэвлэсэн: (2006)

Mating behaviour and female mate choice in the Harvest mouse (Micromys minutus)
-н: Brandt, R
Хэвлэсэн: (2010)

Way-marking behaviour: an aid to spatial navigation in the wood mouse (Apodemus sylvaticus)
-н: Stopka, P, зэрэг
Хэвлэсэн: (2003)

Drosophila Uri, a PPIα binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity
-н: Kirchner, J, зэрэг
Хэвлэсэн: (2008)

Bioinformatical dissection of fission yeast DNA replication origins
-н: Koji Masuda, зэрэг
Хэвлэсэн: (2020-07-01)

Fission-fusion dynamics: new research frameworks
-н: Aureli, F, зэрэг
Хэвлэсэн: (2008)

The impact of intraguild competition with lion Panthera leo on leopard Panthera pardus behavioural ecology
-н: du Preez, B
Хэвлэсэн: (2014)

X-autosome and X-Y translocations in female carriers: X-chromosome inactivation easily detectable by 5-ethynyl-2-deoxyuridine (EdU)
-н: Donat M, зэрэг
Хэвлэсэн: (2017-06-01)

New Caledonia crows use tools for non-foraging activities
-н: Wimpenny, J, зэрэг
Хэвлэсэн: (2011)

Demography and life history of the Eurasian beaver Castor fiber
-н: Campbell, R, зэрэг
Хэвлэсэн: (2010)

Identification and characterisation of five novel Miniature Inverted-repeat Transposable Elements (MITEs) in amphioxus (Branchiostoma floridae)
-н: Osborne, P, зэрэг
Хэвлэсэн: (2006)

Global data for ecology and epidemiology: a novel algorithm for temporal Fourier processing MODIS data
-н: Scharlemann, J, зэрэг
Хэвлэсэн: (2008)

Interplay of population genetics and dynamics in the genetic control of mosquitoes
-н: Alphey, N, зэрэг
Хэвлэсэн: (2014)