Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine
We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...
| Hauptverfasser: | , |
|---|---|
| Format: | Journal article |
| Sprache: | English |
| Veröffentlicht: |
Oxford University Press
2011
|
| Schlagworte: |