Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5'-end, the 3'-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective wi...
Autori principali: | May, J, Brown, L, van Delft, I, Thelwell, N, Harley, K, Brown, T |
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Natura: | Journal article |
Lingua: | English |
Pubblicazione: |
2005
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