Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers
Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson’s disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (A...
| Main Authors: | , , |
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| Format: | Book section |
| Language: | English |
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Springer
2019
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| _version_ | 1797109580384174080 |
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| author | Roberts, RF Bengoa-Vergniory, N Alegre-Abarrategui, J |
| author_facet | Roberts, RF Bengoa-Vergniory, N Alegre-Abarrategui, J |
| author_sort | Roberts, RF |
| collection | OXFORD |
| description | Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson’s disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.
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| first_indexed | 2024-03-07T07:43:39Z |
| format | Book section |
| id | oxford-uuid:40185df3-07e5-4239-9d8e-eeece11478a1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:43:39Z |
| publishDate | 2019 |
| publisher | Springer |
| record_format | dspace |
| spelling | oxford-uuid:40185df3-07e5-4239-9d8e-eeece11478a12023-05-19T09:40:17ZAlpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomersBook sectionhttp://purl.org/coar/resource_type/c_3248uuid:40185df3-07e5-4239-9d8e-eeece11478a1EnglishSymplectic ElementsSpringer2019Roberts, RFBengoa-Vergniory, NAlegre-Abarrategui, JAlpha-synuclein oligomers are thought to be toxic mediators of Parkinson’s disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed. |
| spellingShingle | Roberts, RF Bengoa-Vergniory, N Alegre-Abarrategui, J Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers |
| title | Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers |
| title_full | Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers |
| title_fullStr | Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers |
| title_full_unstemmed | Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers |
| title_short | Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers |
| title_sort | alpha synuclein proximity ligation assay as pla in brain sections to probe for alpha synuclein oligomers |
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