Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1.
The acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level...
| Asıl Yazarlar: | , , |
|---|---|
| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
1988
|
| _version_ | 1826269065898885120 |
|---|---|
| author | Turner, M Chantry, D Feldmann, M |
| author_facet | Turner, M Chantry, D Feldmann, M |
| author_sort | Turner, M |
| collection | OXFORD |
| description | The acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level of mRNA as IL-1 beta mRNA is more abundant than IL-1 alpha mRNA. Studies of transcription in isolated nuclei however indicate that each gene is transcribed at a similar rate, suggesting that post-transcriptional mechanisms regulate the relative abundance of IL-1 alpha and IL-1 beta mRNA. Measurement of RNA half life after addition of alpha-amanitin (an inhibitor of RNA polymerase II) indicate that IL-1 alpha mRNA is not as stable as IL-1 beta mRNA suggesting one mechanism for the different relative levels of RNA. |
| first_indexed | 2024-03-06T21:19:14Z |
| format | Journal article |
| id | oxford-uuid:40debc77-fa1e-4d13-a6cb-39008f1aa7fd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:19:14Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:40debc77-fa1e-4d13-a6cb-39008f1aa7fd2022-03-26T14:40:22ZPost-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:40debc77-fa1e-4d13-a6cb-39008f1aa7fdEnglishSymplectic Elements at Oxford1988Turner, MChantry, DFeldmann, MThe acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level of mRNA as IL-1 beta mRNA is more abundant than IL-1 alpha mRNA. Studies of transcription in isolated nuclei however indicate that each gene is transcribed at a similar rate, suggesting that post-transcriptional mechanisms regulate the relative abundance of IL-1 alpha and IL-1 beta mRNA. Measurement of RNA half life after addition of alpha-amanitin (an inhibitor of RNA polymerase II) indicate that IL-1 alpha mRNA is not as stable as IL-1 beta mRNA suggesting one mechanism for the different relative levels of RNA. |
| spellingShingle | Turner, M Chantry, D Feldmann, M Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. |
| title | Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. |
| title_full | Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. |
| title_fullStr | Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. |
| title_full_unstemmed | Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. |
| title_short | Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. |
| title_sort | post transcriptional control of il 1 gene expression in the acute monocytic leukemia line thp 1 |
| work_keys_str_mv | AT turnerm posttranscriptionalcontrolofil1geneexpressionintheacutemonocyticleukemialinethp1 AT chantryd posttranscriptionalcontrolofil1geneexpressionintheacutemonocyticleukemialinethp1 AT feldmannm posttranscriptionalcontrolofil1geneexpressionintheacutemonocyticleukemialinethp1 |