Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors
In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Humana Press Inc.
2014
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