Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors
In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...
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| Hlavní autoři: |
Bird, L,
Rada, H,
Flanagan, J,
Diprose, J,
Gilbert, R,
Owens, R |
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| Médium: | Journal article
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| Jazyk: | English |
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| Vydáno: |
Humana Press Inc.
2014
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