Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...

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Κύριοι συγγραφείς: Bird, L, Rada, H, Flanagan, J, Diprose, J, Gilbert, R, Owens, R
Μορφή: Journal article
Γλώσσα:English
Έκδοση: Humana Press Inc. 2014
Περιγραφή
Περίληψη:In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. © Springer Science+Business Media New York 2014.