Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

Čájet eará veršuvnnaid (1)

In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...

Bibliográfalaš dieđut
Váldodahkkit:	Bird, L, Rada, H, Flanagan, J, Diprose, J, Gilbert, R, Owens, R
Materiálatiipa:	Journal article
Giella:	English
Almmustuhtton:	Humana Press Inc. 2014

Geahča maid

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.
Dahkki: Bird, L, et al.
Almmustuhtton: (2014)

The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.
Dahkki: Berrow, N, et al.
Almmustuhtton: (2009)

Design and cloning strategies for constructing shRNA expression vectors
Dahkki: McIntyre Glen J, et al.
Almmustuhtton: (2006-01-01)

GST Fusion Protein Expression Vector for In-Frame Cloning and Site-Directed Mutagenesis
Dahkki: Thérèse Hunter, et al.
Almmustuhtton: (1998-02-01)

Construction of an Escherichia coli export-affinity vector for expression and purification of foreign proteins by fusion to cyclomaltodextrin glucanotransferase /
Dahkki: 383265 Hellman, Jukka, et al.

Development of a novel vector for cloning and expressing extremely toxic genes in Escherichia coli
Dahkki: Hedan Li, et al.
Almmustuhtton: (2017-11-01)

Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination.
Dahkki: Jeongmin Lee, et al.
Almmustuhtton: (2013-01-01)

Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.
Dahkki: M. А. Gracheva, et al.
Almmustuhtton: (2007-12-01)

Improved Cloning Vectors for Transgene Construction
Dahkki: Yinqing Yang, et al.
Almmustuhtton: (1997-06-01)

Simplified screening for the detection of soluble fusion constructs expressed in <it>E. coli </it>using a modular set of vectors
Dahkki: Lawrence Ann-Marie, et al.
Almmustuhtton: (2005-12-01)

Using a Eukaryotic GST Fusion Vector for Proteins Difficult to Express in E. coli
Dahkki: Robert Y.L. Tsai, et al.
Almmustuhtton: (1997-11-01)

Cloning and Expression Vector Construction of Glutamate Decarboxylase Gene from Lactobacillus Plantarum
Dahkki: B Arabpour, et al.
Almmustuhtton: (2016-06-01)

Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
Dahkki: Che Nordin, Muhamad Alif
Almmustuhtton: (2017)

Recombinational cloning vectors for regulated expression in Saccharomyces cerevisiae
Dahkki: John R. Geiser
Almmustuhtton: (2005-03-01)

Cloning vectors for expression-PCR products
Dahkki: Jean-Michel Betton
Almmustuhtton: (2004-09-01)

Mammalian Expression Vectors for Epitope Tag Fusion Proteins that Are Toxic in E. coli
Dahkki: Rolf Jakobi, et al.
Almmustuhtton: (2002-12-01)

Recombinant Expressed Vector pET32a (+) S Constructed by Ligation Independent Cloning
Dahkki: Yu Wang, et al.
Almmustuhtton: (2014-10-01)

Interleukin gene cloning and expression in E. coli
Dahkki: Filipa Oliveira, et al.
Almmustuhtton: (2021-04-01)

High-throughput cloning, expression, and purification
Dahkki: Owens, R, et al.
Almmustuhtton: (2007)

Molecular Cloning of DARPins G3 in pET28b Expression Vector and Optimization of the Expression of This Protein in Escherichia Coli
Dahkki: Nadereh Bakhshandeh, et al.
Almmustuhtton: (2022-03-01)

Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet
Dahkki: FAN Ziqi, et al.
Almmustuhtton: (2025-01-01)

Novel Cloning Method for Recombinant Adenovirus Construction in Escherichia coli
Dahkki: David W. Souza, et al.
Almmustuhtton: (1999-03-01)

Cloning of Bacillus subtilis phytase gene construct in Escherichia coli
Dahkki: Mahdiyar Iravani Saadi, et al.
Almmustuhtton: (2021-10-01)

Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli
Dahkki: Xiaoliang HE, et al.
Almmustuhtton: (2019-04-01)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.
Dahkki: Trikkur Madom Seetaraman Amritha, et al.
Almmustuhtton: (2020-01-01)

Cloning of the filamentous hemagglutinin of bordetella pertussis and its expression in escherichia coli /
Dahkki: 377857 Brown, Douglas R., et al.

Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector
Dahkki: Lele Li, et al.
Almmustuhtton: (2022-11-01)

Mammalian Expression Vector with Two Multiple Cloning Sites for Expression of Two Foreign Genes
Dahkki: T.C. Tsang, et al.
Almmustuhtton: (1997-01-01)

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.
Dahkki: Berrow, N, et al.
Almmustuhtton: (2007)

Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli
Dahkki: Bettina Rohweder, et al.
Almmustuhtton: (2018-01-01)

Highly efficient yeast-based in vivo DNA cloning of multiple DNA fragments and the simultaneous construction of yeast/Escherichia coli shuttle vectors
Dahkki: Ei′ichi Iizasa, et al.
Almmustuhtton: (2006-01-01)

Cloning of fusion protein gene of Newcastle disease virus into a baculovirus derived bacmid shuttle vector, in order to express it in insect cell line
Dahkki: Hashemzadeh MS, et al.
Almmustuhtton: (2015-05-01)

Construction of new ligation-independent cloning vectors for the expression and purification of recombinant proteins in silkworms using BmNPV bacmid system.
Dahkki: Tatsuya Kato, et al.
Almmustuhtton: (2013-01-01)

Cloning and expression of human APOBEC3B in Escherichia Coli /
Dahkki: Ahmed Bouna, 1984-, author 639404, et al.
Almmustuhtton: (2016)

Cloning, Expression and Characterization of PprI gene in Escherichia coli
Dahkki: Amir Mirzaie, et al.
Almmustuhtton: (2014-03-01)

Cloning and expression soluble recombinant parathyroid hormone in E. coli
Dahkki: Aghajani MH, et al.
Almmustuhtton: (2015-12-01)

Construction of fluorescent human zona pellucida fusion protein expression vectors and expression in CHO cells
Dahkki: WANG Mingyi, ZHU Yan
Almmustuhtton: (2021-12-01)

Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System
Dahkki: Abbas Mohajeri, et al.
Almmustuhtton: (2016-06-01)

New E. coli Cloning Vector Using a Cellulase Gene (celA) as a Screening Marker
Dahkki: S.J. Jang, et al.
Almmustuhtton: (2001-11-01)

Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from <it>Mycobacterium tuberculosis</it>
Dahkki: Challa Shridhar V, et al.
Almmustuhtton: (2008-05-01)