Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

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In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in E...

書目詳細資料
Main Authors:	Bird, L, Rada, H, Flanagan, J, Diprose, J, Gilbert, R, Owens, R
格式:	Journal article
語言:	English
出版:	Humana Press Inc. 2014

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