Ratiometric fluorescence-imaging assays of plant membrane traffic using polyproteins.
Fluorescent protein markers are widely used to report plant membrane traffic; however, effective protocols to quantify fluorescence or marker expression are lacking. Here the 20 residue self-cleaving 2A peptide from Foot and Mouth Disease Virus was used to construct polyproteins that expressed a tra...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2006
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