Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot: Variability of the memory B cell ELISpot assay.

Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot: Variability of the memory B cell ELISpot assay.

The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (BMEM), and can be performed using cryopreserved samples. The effects of cryopreservation on BMEM detection and the consistency of cultured ELISpot assays when performed by different operators or l...

Полное описание

Библиографические подробности
Главные авторы:	Trück, J, Mitchell, R, Thompson, A, Morales-Aza, B, Clutterbuck, E, Kelly, D, Finn, A, Pollard, A
Формат:	Journal article
Язык:	English
Опубликовано:	2014

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