Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.

The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+ -releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchlori...

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Bibliographic Details
Main Authors: Lewis, A, Masgrau, R, Vasudevan, SR, Yamasaki, M, O'Neill, J, Garnham, C, James, K, Macdonald, A, Ziegler, M, Galione, A, Churchill, G
Format: Journal article
Language:English
Published: 2007