Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.
The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+ -releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchlori...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2007
|
| _version_ | 1826269504553877504 |
|---|---|
| author | Lewis, A Masgrau, R Vasudevan, SR Yamasaki, M O'Neill, J Garnham, C James, K Macdonald, A Ziegler, M Galione, A Churchill, G |
| author_facet | Lewis, A Masgrau, R Vasudevan, SR Yamasaki, M O'Neill, J Garnham, C James, K Macdonald, A Ziegler, M Galione, A Churchill, G |
| author_sort | Lewis, A |
| collection | OXFORD |
| description | The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+ -releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchloric acid and then measure the NAADP using a radioreceptor assay. We demonstrate that NAADP is neither generated nor broken down during sample processing conditions and that radioreceptor assay is highly selective for the detection of NAADP under cell extract conditions. Furthermore, a number of improvements, such as solid-state detection of the radioactivity, are incorporated to enhance the safety of the procedure. Finally, we have developed a new method to prevent the endogenous metabolism of NAADP by chelating Ca2+ with bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), thereby reducing the difficulty of catching a small transient rise in NAADP levels. In summary, we have refined and improved a method for measuring NAADP levels and presented it in a manner accessible to a wide range of laboratories. It is expected that this will enhance research in the NAADP field. |
| first_indexed | 2024-03-06T21:26:06Z |
| format | Journal article |
| id | oxford-uuid:43236c7a-0094-494b-a219-0f466d42c33d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:26:06Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:43236c7a-0094-494b-a219-0f466d42c33d2022-03-26T14:53:42ZRefinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:43236c7a-0094-494b-a219-0f466d42c33dEnglishSymplectic Elements at Oxford2007Lewis, AMasgrau, RVasudevan, SRYamasaki, MO'Neill, JGarnham, CJames, KMacdonald, AZiegler, MGalione, AChurchill, GThe measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+ -releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchloric acid and then measure the NAADP using a radioreceptor assay. We demonstrate that NAADP is neither generated nor broken down during sample processing conditions and that radioreceptor assay is highly selective for the detection of NAADP under cell extract conditions. Furthermore, a number of improvements, such as solid-state detection of the radioactivity, are incorporated to enhance the safety of the procedure. Finally, we have developed a new method to prevent the endogenous metabolism of NAADP by chelating Ca2+ with bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), thereby reducing the difficulty of catching a small transient rise in NAADP levels. In summary, we have refined and improved a method for measuring NAADP levels and presented it in a manner accessible to a wide range of laboratories. It is expected that this will enhance research in the NAADP field. |
| spellingShingle | Lewis, A Masgrau, R Vasudevan, SR Yamasaki, M O'Neill, J Garnham, C James, K Macdonald, A Ziegler, M Galione, A Churchill, G Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate. |
| title | Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate. |
| title_full | Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate. |
| title_fullStr | Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate. |
| title_full_unstemmed | Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate. |
| title_short | Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate. |
| title_sort | refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate |
| work_keys_str_mv | AT lewisa refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT masgraur refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT vasudevansr refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT yamasakim refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT oneillj refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT garnhamc refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT jamesk refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT macdonalda refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT zieglerm refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT galionea refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate AT churchillg refinementofaradioreceptorbindingassayfornicotinicacidadeninedinucleotidephosphate |