Mapping sequences in loops of nuclear DNA by their progressive detachment from the nuclear cage.
Nuclear DNA is organised into loops, probably by attachment to a supramolecular structure. We describe a method which enables us to map the position of sequences within a loop relative to the point of attachment. Nuclear DNA is isolated unbroken by lysing HeLa cells in 2M NaCl to release structures...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1980
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