Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde.
(Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, inc...
| 主要な著者: | , , , , |
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| フォーマット: | Journal article |
| 言語: | English |
| 出版事項: |
2010
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| 要約: | (Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme-catalysed demethylation. © 2010 Wiley-VCH Verlag GmbHand Co. KGaA. |
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