DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement.
Centrioles form cilia and centrosomes, organelles whose dysfunction is increasingly linked to human disease. Centriole duplication relies on a few conserved proteins (ZYG-1/Sak/Plk4, SAS-6, SAS-5/Ana2, and SAS-4), and is often initiated by the formation of an inner "cartwheel" structure. H...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1826270805977202688 |
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| author | Stevens, N Roque, H Raff, J |
| author_facet | Stevens, N Roque, H Raff, J |
| author_sort | Stevens, N |
| collection | OXFORD |
| description | Centrioles form cilia and centrosomes, organelles whose dysfunction is increasingly linked to human disease. Centriole duplication relies on a few conserved proteins (ZYG-1/Sak/Plk4, SAS-6, SAS-5/Ana2, and SAS-4), and is often initiated by the formation of an inner "cartwheel" structure. Here, we show that overexpressed Drosophila Sas-6 and Ana2 coassemble into extended tubules (SAStubules) that bear a striking structural resemblance to the inner cartwheel of the centriole. SAStubules specifically interact with centriole proximal ends, but extra DSas-6/Ana2 is only recruited onto centrioles when Sak/Plk4 kinase is also overexpressed. This extra centriolar DSas-6/Ana2 induces centriole overduplication and, surprisingly, increased centriole cohesion. Intriguingly, we observe tubules that are structurally similar to SAStubules linking the engaged centrioles in normal wild-type cells. We conclude that DSas-6 and Ana2 normally cooperate to drive the formation of the centriole inner cartwheel and that they promote both centriole duplication and centriole cohesion in a Sak/Plk4-dependent manner. |
| first_indexed | 2024-03-06T21:46:36Z |
| format | Journal article |
| id | oxford-uuid:49d08b21-78eb-42f1-bc88-7e8d92967933 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:46:36Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:49d08b21-78eb-42f1-bc88-7e8d929679332022-03-26T15:33:50ZDSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:49d08b21-78eb-42f1-bc88-7e8d92967933EnglishSymplectic Elements at Oxford2010Stevens, NRoque, HRaff, JCentrioles form cilia and centrosomes, organelles whose dysfunction is increasingly linked to human disease. Centriole duplication relies on a few conserved proteins (ZYG-1/Sak/Plk4, SAS-6, SAS-5/Ana2, and SAS-4), and is often initiated by the formation of an inner "cartwheel" structure. Here, we show that overexpressed Drosophila Sas-6 and Ana2 coassemble into extended tubules (SAStubules) that bear a striking structural resemblance to the inner cartwheel of the centriole. SAStubules specifically interact with centriole proximal ends, but extra DSas-6/Ana2 is only recruited onto centrioles when Sak/Plk4 kinase is also overexpressed. This extra centriolar DSas-6/Ana2 induces centriole overduplication and, surprisingly, increased centriole cohesion. Intriguingly, we observe tubules that are structurally similar to SAStubules linking the engaged centrioles in normal wild-type cells. We conclude that DSas-6 and Ana2 normally cooperate to drive the formation of the centriole inner cartwheel and that they promote both centriole duplication and centriole cohesion in a Sak/Plk4-dependent manner. |
| spellingShingle | Stevens, N Roque, H Raff, J DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. |
| title | DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. |
| title_full | DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. |
| title_fullStr | DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. |
| title_full_unstemmed | DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. |
| title_short | DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. |
| title_sort | dsas 6 and ana2 coassemble into tubules to promote centriole duplication and engagement |
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